Equine immunology – why do we need vaccines for horses?
Stuart Davies BVSc MRCVS, explains how and why vaccines for horses work
As horse owners we strive to maintain our horses in good health. One of the main ways we do this is by vaccinating against infectious pathogens. A pathogen is a bacteria, virus or fungi that can cause disease. While there are many vaccines available for different diseases, who actually knows what, why or how this vaccine is producing immunity? In the following article we will look at the development of the horse’s immune system and how vaccines produce protection.
How do vaccines work?
There are two pathways of the immune system that work together to protect the body from infection:
The innate immune system
The innate immune system is non-specific and comprises of anatomical, such as skin and mucous membranes, physiological, phagocytic and inflammatory barriers. Pattern recognition receptors (PRRs) that recognise common structures shared between molecules such as cell walls and viral RNA are integral to successful function. Upon recognition, the innate immune system rapidly recruits immune cells to sites of infection or inflammation from the release of chemical signalling molecules called cytokines. This then starts a cascade of chemical reactions both locally, in the tissue affected, and body wide to rid the pathogen from the body. Macrophage cells can engulf the pathogen, break it down and also present the antigen, a marker on the surface that enables the body to recognise it, to a T-cell.
The adaptive immune system
The next pathway is the adaptive immune system. This enables the body to recognise specific antigens and distinguish from itself, target their removal in specific ways and, produce memory so that if reinfection occurs the body can recognise it swiftly. T cells are produced in bone marrow and circulate throughout the body in lymph fluid, a fluid that hydrates cells. When the antigen is presented to a T cell it becomes an effector cell, degrading the pathogen and removing it from the body. However, some effector cells remain within the body and are now called memory cells. Memory cells can turn back into effector cells when re-stimulated by repeat exposure of the same antigen.
An additional bone marrow derived cell involved in the adaptive immune system is the B cell. These do not need macrophages to present the antigen but recognise the antigen directly and, degrade the pathogen with the production of antibodies, as well as producing long lived memory cells. The T cells are responsible for cell mediated immunity whereas B cells are responsible for antibody mediated immunity.
How is immunity acquired?
Immunity can be acquired from exposure to antigens in the environment. The foal is an example of this – it has come from a safe uterus into a world of novel antigens, which its body will have to recognise, determine if a risk, breakdown and get rid of, in addition to then remembering it for the future. This is an example of active immunisation.
This same process happens when we vaccinate an animal but, instead of using a pathogen able to produce infection it is often attenuated (weakened), inactivated or, only part of the pathogen. Foals have been shown to produce antibodies in utero. However, their immune system is not fully developed when born and in the first few weeks of life.
A mare’s placenta actually prevents the transfer of the dam’s antibodies to the foal. Therefore, to help the foal deal with its environment, passive immunity is provided by the transfer of antibodies in colostrum. Colostrum is produced by the mare, and only absorbed by the foal’s gut in the first 12-24 hours of life. The antibody that predominates in colostrum is IgG.
Foals deficient in colostrum intake have been shown to be at a greater risk of infection. Therefore, it is commonplace for vets to perform an IgG test on a blood sample of a foal at roughly a day of age. If there has been failure of passive transfer of maternally derived antibodies, either through inadequate colostrum of insufficient intake, then an infusion of hyperimmune plasma can be administered. The plasma given can be enriched with antibodies targeted against a specific pathogen such as Rhodococcus equi, a bacterial pathogen associated with pneumonia in foals.
Colostral antibodies, while helping the foal during immune system development, have been suggested to affect the success of vaccinations at a young age. Foals who received colostrum failed to produce antibodies following influenza vaccination until 6-8 months of age – when colostral antibodies declined. However, this mechanism is not fully understood. The same does not occur in adult horses who receive an influenza booster when they already have influenza antibodies from exposure or previous vaccination.
Vaccinations must be given with specific time periods between primary vaccination courses however, these time periods vary between the governing bodies of different countries. A protective level of antibodies against the disease vaccinated for has been established by studies and, failed to be achieved after just one vaccine. Interestingly, the window for 2nd vaccination for Equine Influenza Virus (EIV) can be 21-91 days after the first vaccination. A greater number of individuals in the 2 and 3 month gap vaccine group, had insufficient antibody levels by the time of 2ndvaccination. However, in delaying the 2nd vaccination till the later period provided a more intense antibody peak, and, an increased time before antibody levels declined prior to booster vaccination a year later.
Vaccines for horses have been shown to reduce the likelihood of infection, spreading of the virus and severity of clinical signs shown when infected. In outbreaks of EIV it becomes apparent that within vaccinated horses the older age group have a greater level of disease. This is due to the larger vaccination interval, often annual if not lapsed, in comparison to two-year olds who have received 4 vaccines within a two-year interval.
Vaccination site reactions
Vaccines are required to be updated to include more modern strains as diseases mutate, so that protection applies to current in field strains. However, some cross protection will be provided, but can’t be guaranteed from using vaccines containing historic strains.
In order to increase the recognition of the vaccine the attenuated, inactivated or, part vaccine, is mixed with an adjuvant. It is the adjuvant, which can cause an injection site reaction. This can present a pyrexia (fever), swelling and discomfort surrounding the injection site. Unfortunately, to reduce the risk of injection site reaction, would consequentially affect bodily recognition and function of the vaccine.
In summary, vaccinations are used to help protect the horse from many different infectious diseases.
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